Cadáveres quimicamente preservados com Solução de Larssen modificada - um método alternativo de conservação
Alternative methods for the use of animals in research and training of students emerged with the aim of reducing the number of animals needed in experiments or refining the methodology in order to reduce the pain or discomfort suffered by them. Objective: The present study analyzed, qualitatively, the use of the modified Larssen solution in animal cadavers, in order to reduce the number of animals for experimentation. Method: 4 pigs were used, after euthanasia, were treated with the modified Larssen's solution and placed in a freezer for cryopreservation. The cadavers were thawed and evaluated six weeks after the procedure, using a previously prepared questionnaire. Results: Eight evaluators performed the evaluation. Regarding the quality of the corpse, six evaluators judged that there was a considerable improvement in the quality of the corpse and two judged a small improvement. Regarding the putrefaction odor, six evaluated that there was a reduction in odor and two reported that there was no change. Regarding the external aspect of the corpse, three evaluators judged the absence of signs of putrefaction and skin changes, three judged the beginning of putrefaction and one judged the absence of signs of putrefaction. Finally, in relation to the possibility of surgical training, six evaluators judged that the corpse under study allows for intense and satisfactory training of all operative maneuvers Conclusion: The conservation technique has proved to be effective and of real importance as it may decrease the use of animals well as the costs for conducting practical classes in vivo.
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